Gao Dan
Assistant Professor

Dr. Gao’s research focuses on: microfluidics-based cell analysis, microdroplet reaction, MALDI imaging, LC-MS based metabolomics, and proteomics. To date, she has published more than 40 papers in Anal Chem, Lab Chip, Trends in Anal Chem, Proteomics and other journals. In the field of microfluidics, she firstly established the microfluidic-mass spectrometry combination system for online cell, cell-cell co-culture, or drug analysis. Compared with conventional chemical reactions, the developed novel microdroplet reaction is highly controllable, the reaction rate is significantly accelerated, and the reaction conditions are extremely mild. Dr. Gao has received funding awards from the Natural Science Foundation of China, the Natural Science Foundation of China (Youth Fund), the Natural Science Foundation of Guangdong Province, and the China Postdoctoral Science Foundation.

Education

July. 2011 Ph. D. in Analytical Chemistry, Department of Chemistry, Beijing University of Chemical Technology

July.2007-July. 2011 united training at Prof. Jin-Ming Lin’s group at Department of Chemistry, Tsinghua University

July. 2012 B.S. in Chemistry, College of Science, Beijing Institute of Fashion Technology

Research Experience

July. 2013-present The Graduate School at Shenzhen, Tsinghua University

Jan. 2018-Jan. 2019 Visiting Scholar, Department of Chemistry, Stanford University

July. 2011-June. 2013 postdoctor, The Graduate School at Shenzhen, Tsinghua University

[1]  Ning Wang, Shaopeng Chen, Bin Zhang, Shangfu Li, Feng Jin, Dan Gao*, Hongxia Liu and Yuyang Jiang, 8u, a pro-apoptosis/cell cycle arrest compound, suppresses invasion and metastasis through HSP90α downregulating and PI3K/Akt inactivation in hepatocellular carcinoma cells, Scientific Reports, 2018, 8, 309.

[2]  Anqi Li, Chunyan Tan*, Tianying Yuan, Jiamei Liang, Dan Gao*, Ying Tan, Yuyang Jiang*, Efficient photocatalytic oxidation sensitized by conjugated polymers in a batch reaction and microreactors under visible light, J. Mater. Chem. A, 2018, 6, 15927-15932.

[3]  Yongli Chen, Dan Gao*, Yanwei Wang, Shuo Lin, Yuyang Jiang, A novel 3D breast-cancer-on-chip platform for therapeutic evaluation of drug delivery systems, Analytica Chimica Acta, 2018, 1036, 97-106.

[4]  Wenrui Liu, Feng Jin, Dan Gao*, Lu Song, Chao Ding and Hongxia Liu*, Metabolomics analysis reveals aminoquinazolin derivative 9d-induced oxidative stress and cell cycle arrest in A549 cells, RSC Advances, 2017, 7, 13149–13158.

[5]  Xiaojian Shao, Dan Gao*, Yongli Chen, Feng Jin, Guangnan Hu, Yuyang Jiang, Hongxia Liu*, Development of a blood-brain barrier model in a membrane-based microchip for characterization of drug permeability and cytotoxicity for drug screening, Analytical Chimica Acta, 2016, 934, 186-193.

[6]      Yini Wang, Dan Gao, Bizhu Chu, Chunmei Gao, Deliang Cao, Hongxia Liu*,  Yuyang Jiang, Exposure of CCRF-CEM cells to acridone derivative 8a triggers tumor death via multiple mechanisms, Proteomics 2016,16, 1177–1190.

[7]     Weiyi Xie, Dan Gao*, Feng Jin, Yuyang Jiang, and Hongxia Liu*. Study of Phospholipids in Single Cells Using an Integrated Microfluidic Device Combined with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry. Analytical Chemistry, 2015, 87, 7052?7059.

[8]     Qin Wu1, Dan Gao1, Juntong Wei, Feng Jin, Weiyi Xie, Yuyang Jiang*, Hongxia Liu*. Development of a novel multi-layer microfluidic device towards characterization of drug metabolism and cytotoxicity for drug screening. Chemical Communications, 2014, 50, 2762-2764. (1The two authors contributed equally to this work)

[9]     Dan Gao, Hongxia Liu, Yuyang Jiang and Jin-Ming Lin*. Recent advances in microfluidics combined with mass spectrometry: technologies and applications. Lab on a Chip, 2013, 13, 3309-3322.

[10]   Dan Gao, Hongxia Liu, Jin-Ming Lin*, Yini Wang and Yuyang Jiang*. Characterization of drug permeability in Caco-2 monolayers by mass spectrometry on a membrane-based microfluidic device, Lab on a Chip, 2013, 13, 978-985.

[11] Dan Gao, Hongxia Liu, Yuyang Jiang and Jin-Ming Lin*. Recent development of microfluidic devices in vitro cell culture for cell biology research, Trends in Analytical Chemistry, 2012, 35, 150–164.

[12]   Dan Gao, Haifang Li, Niejun Wang, and Jin-Ming Lin*. Evaluation of the Absorption of Methotrexate on Cells and Its Cytotoxicity Assay by Using an Integrated Microfluidic Device Coupled to a Mass Spectrometer, Analytical Chemistry, 2012, 84, 9230-9237.

[13]   Dan Gao, Huibin Wei, Guang-Sheng Guo, and Jin-Ming Lin*. Microfluidic Cell Culture and Metabolism Detection with Electrospray Ionization Quadrupole Time-of-Flight Mass Spectrometer, Analytical Chemistry, 2010, 82, 5679-5685.

[14]   Dan Gao, Jiangjiang Liu, Hui-Bin Wei, Hai-Fang Li, Guang-Sheng Guo, Jin-Ming Lin*. A Microfluidic Approach for Anticancer Drug Analysis Based on Hydrogel Encapsulated Tumor Cells, Analytica Chimica Acta, 2010, 665, 7–14.

[15]      Dan Gao, Hai-Fang Li, Guang-Sheng Guo, and Jin-Ming Lin*. Magnetic Bead Based Immunoassay for Enumeration of CD4+ T Lymphocytes on a Microfluidic Device, Talanta, 2010, 82, 528-533.